(Mucopeptide N-acetylmuramylhydrolase; EC 3.2.1.17)
Lysozyme catalyzes the hydrolysis of §-1,4 glucosidic linkages which occur in the cell walls of microorganisms. It is widely distributed in animals and plants. Lysozyme from chicken egg white has been extensively studied. It is a basic protein with a molecular weight of approximately 14,000.
Lysozyme is normally present in plasma (5.9 mg/l) but only in trace amounts in urine. In certain renal disorders, urinary excretion of lysozyme is significantly increased, which could be of diagnostic significance (Harrison, J.F.; Parker, R.W. and De Silva, K.L., J. Clin. Pathol., 26, 278, 1973). Analysis of serum lysozyme levels could also be used as a diagnostic tool in acute and chronic myelocytic leukemia and in acute lymphocytic leukemia (Grossowicz, N.; Ariel, M. and Weber, T., Clin. Chem., 25, 484, 1979).
1.Source: | Chicken Egg White (Ezymatic Grade) |
Form: | Freeze-dried |
Solubility: | Soluble in distilled water or dilute buffer |
Stability: | -20° C; -4° F |
Activity (approx.): | 50,000 U/mg |
Protein (approx.): | 95% (biuret) |
Catalog No.: | EN-11-097A50000 |
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That amount of enzyme causing a decrease in absorbance at 450 nm of 0.001 per minute at 25°C and pH 6.24 with Micrococcus lysodeikticus as substrate.
The rate of lysis of Micrococcus lysodeikticus, catalyzed by lysozyme, is proportional to the decrease in extinction at 450 nm (Shugar, D., Biochim, Biophys. Acta, 8, 302, 1952).